A Laboratory-Based Experimental Study on the Protective Effects Against Isoniazid- Induced Hepatotoxicity in Mice

Abstract

Objective: To evaluate the role of Alpha Lipoic Acid (ALA) as a potential antidote to isoniazid-induced hepatitis.
Study Design: Laboratory-based experimental animal study.
Place and Duration of Study: The study was conducted at the Department of Pharmacology, Army Medical
College, Rawalpindi and The National Institute of Health, Islamabad, Pakistan from January 2020 to June 2021.
The study was designed and reported in compliance with the ARRIVE (Animal Research: Reporting of in Vivo
Experiments) guidelines.
Methods: A total of 90 adult male healthy mice were obtained from NIH and were kept at the animal house of
NIH, Islamabad under standard conditions with a daily photoperiod of 12 hours light and 12 hours dark at temp
22 30C. The mice were acclimatized to the laboratory conditions for 1 week, prior to experimentation, and were
provided with a standard diet and water ad libitum. Male mice having a weight of 30-50 grams were included in
the study. Mice having deranged liver function tests at the start of the experimentation were excluded. Mice
were equally divided into three groups. In group A, hepatotoxicity was induced by administration of 100 mg/kg
of oral isoniazid, which was indicated by elevated liver enzymes and changes in histo-pathological parameters.
Alpha-lipoic acid (ALA) was administered orally at 50mg/kg 1 hour prior to isoniazid for 28 days. Animals were
sacrificed on the 29^th day, and samples of blood and liver were taken for biochemical and histopathological
analysis.
Results: Results of this study indicated that ALA offered protection against isoniazid-induced hepatotoxicity by
reducing levels of bilirubin and liver enzymes in the serum. Hepato-protection was further evident from the
preservation of liver architecture, which was indicated by reduced necrosis, steatosis, and portal inflammation.
Conclusion: Alpha-lipoic acid (ALA) proved to be effective in protecting against isoniazid-induced
hepatotoxicity.

How to cite this: Khan IU, Waheed A, Khan WU, Aslam J, Mehmood MS, Khan BU. A Laboratory-Based Experimental Study on the Protective Effects Against Isoniazid-Induced Hepatotoxicity in Mice. Life and Science. 2025; 6(4): 471-476. doi: http://doi.org/10.37185/LnS.1.1.784