Protective Effect of Pyridoxine on Lead Nitrate Induced Histomorphological Changes in Rat Liver

Sumaira Abbasi, Huma Musarrat Khan, Muhammad Yunus Khan

ABSTRACT

Objective: To study the effects of Pyridoxine on lead nitrate generated histological and morphological changes in rat liver.
Study Design: It was an experimental study.
Place and Duration of Study: The study was carried out at Anatomy Department of College of Physicians and Surgeons, Islamabad, Pakistan from 03rd September, 2019 to 31st December, 2020.
Materials and Methods: In this study 90 male rats were used and divided into three groups. Control group A was kept on normal diet. Experimental group B1 was given lead nitrate and B2 was given lead nitrate and pyridoxine by oral gavage for a period of 2 weeks. All rats were sacrificed after 2 weeks. Results of quantitative variables such as inflammatory and Kupffer cells were expressed in mean ± S.D. To detect significant difference among the groups, ANOVA accompanied by Post Hoc Tukey test was applied and qualitative data such as central vein congestion, steatosis, and necrosis were expressed in percentages and Chi Square test.
Results: Exposure to pyridoxine reduces the degenerative changes caused by lead nitrate such as increase in the number of Kupffer and inflammatory cells. Central vein congestion, steatosis and necrotic foci were also reduced with a significant p-value.
Conclusion: An ameliorative effect of pyridoxine on the degenerative changes in the liver induced by lead nitrate through a decrease in the number of Kupffer and inflammatory cells. Central vein congestion, steatosis and necrotic foci were also reduced significantly.

Keywords: Congestion, Free radicals, Lead nitrate, Necrosis, Oxidative stress, Pyridoxine and Steatosis.

How to cite this: Abbasi S, Khan HM, Khan MY.  Protective Effect of Pyridoxine on Lead Nitrate Induced Histomorphological Changes in Rat Liver. Life and Science. 2022; 3(4): 163-167. doi: http://doi.org/10.37185/LnS.1.1.269

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